Open AccessINDUCTION OF UNDIFFERENTIATED TUMORS BY JC VIRUS IN THE CEREBRUM OF RATS
Author(s) -
Ohsumi Shozo,
Motoi Makoto,
Ogawa Katsuo
Publication year - 1986
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1986.tb03116.x
Subject(s) - cerebrum , pathology , biology , anatomy , medicine , neuroscience , central nervous system
Newborn Sprague‐Dawley rats were inoculated intracranially with JC virus (Tokyo‐1), a human polyomavirus, which had been isolated by N agashima et al. from the autopsied brain of a patient with progressive multifocal leukoencephalopathy in Japan. Twenty‐one to 70 weeks later, 21 of 27 rats developed brain tumors in the cerebrum, but not in the cerebellum. Most of the tumor cells were of an undifferentiated neuroectodermal nature and showed nuclear palisades and pseudorosettes. In some tumor cells glial flbrillary acidic protein was positive immunohistochemically, and many glial filaments were demonstrated ultrastructurally. Neuronal differentiation was not proved. Two continuous lines of cultured tumor cells were established, and T antigen of JCV (Tokyo‐1) was present in both cell lines. Glial differentiation was confirmed also in the tumors produced by subcutaneous transplantation of cultured tumor cells.