Open AccessESTABLISHMENT OF MONOCLONAL ANTIBODIES REACTIVE WITH EPITHELIAL and MYOEPITHELIAL CELLS IN THE BREAST
Author(s) -
Imai Shunsuke,
Sekiya Nao,
Mobimoto Junji,
Kiyozuka Yasuhiko,
Tsubura Yoshihiko,
Sakamoto Goi
Publication year - 1986
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1986.tb02852.x
Subject(s) - myoepithelial cell , monoclonal antibody , pathology , immunohistochemistry , antibody , monoclonal , biology , medicine , immunology
Monoclonal antibodies which are considered to be able to differentiate epithelial and myoepithelial cells in the breast have been developed. Human mammary carcinoma cell line (HBC‐4W) was used for immunization. Monoclonal antibodies‐B4B2F10 (epi‐1), E9E8B7 (myo‐l)‐with IgM was examined using tissues from diseased breast by avidin‐biotin‐peroxidase assay. Epi‐1 antibody reacted with epithelial cells while myo‐1 antibody reacted with myoepithelial cells in the mammary glands, respectively. The reaction was markedly visible, in particular, in fibroadenoma, mastopathy, and papilloma, which showed clear two‐cell‐type structures. In the infiltrating ductal carcinoma, epi‐1 antibody reacted with carcinoma cells, while myo‐1 antibody reacted with stromal cells rather than carcinoma cells suggesting that infiltrating ductal carcinoma was mainly of epithelial origin. In the infiltrating lobular carcinoma, however, myo‐1 as well as epi‐1 antibodies reacted with carcinoma cells. It is suggested that infiltrating lobular carcinoma was of a mixture of epithelial and myoepithelial cell origin. ACTA PATHOL. JPN. 36: 1319–1326, 1986.