Open AccessPARTICIPATION OF BONE MARROW STROMAL CELLS IN HEMOPOIETIC RECOVERY OF RATS IRRADIATED AND THEN PARABIOSED WITH A NON‐IRRADIATED LITTER MATE
Author(s) -
Kagawa Koichi,
Hayashi Keiki,
Awai Michiyasu
Publication year - 1986
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1986.tb00211.x
Subject(s) - litter , stromal cell , bone marrow , haematopoiesis , irradiation , pathology , biology , medicine , stem cell , microbiology and biotechnology , ecology , physics , nuclear physics
A light microscopical study on the recovery process after lethal irradiation and parabiosis has been made. 6 Electron microscopically, in the bone marrow of lethally irradiated rats, hemorrhage occurred due to detachment of sinus endothelial cells. Afterwards, reticulum cells with small intracytoplasmic lipid droplets appeared. On day 3, these cells were rapidly replaced by the reticulum cells with large lipid droplets, and resulted in fatty marrow within 7 days. Spindle‐shaped fibroblastoid reticulum cells were also observed. In the bone marrow of lethally irradiated rats parabiosed with non‐treated litter mates, hemopoiesis was initiated by adhesion of nucleated blood cells to intricated fine cytoplasmic pseudopods of fat‐storage cells. On days 3 to 5, in parallel with progressive hemopoietic recovery, flbroblastoid and reticulum cells with large lipid droplets decreased whereas those with small droplets increased. On day 8, reticulum cells with lipid droplets were seldom seen, and hemopoietic distribution became the same as normal. These results suggested that bone marrow stromal cells, namely reticulum, fat‐storage, and flbroblastoid cells share a common cellular origin, and also that they regain their structure and function when fat‐storage cells were placed in contact with hemopoietic precursor cells.