Open AccessPREPARATION OF MONOCLONAL ANTIBODY TO HEPATOCELLULAR MEMBRANES AND ITS APPLICATION TO INDUCTION OF LIVER CELL MEMBRANE DAMAGE
Author(s) -
Sato Keiki,
Ikeda Takuya,
Katami Kazuei,
Ogawa Hidemasa
Publication year - 1985
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1985.tb01435.x
Subject(s) - monoclonal antibody , antibody , microbiology and biotechnology , immunofluorescence , hepatocyte , cell , chemistry , membrane , complement system , biology , pathology , immunology , biochemistry , in vitro , medicine
Monoclonal antibody (MoAb) to rat liver plasma membranes was prepared by hybridization of mouse immune lymphocytes with mouse myeloma cells, and was identified by the immunodiffusion method in a fraction of IgM secreted from the hybridoma thus obtained. In indirect immunofluorescence tests, specific fluorescence was detected only on the surface of rat hepatocytes, but neither on the cells from other organs of the rat nor on the hepatocytes of other species of animals, suggesting that the antibody may be organ‐ and species‐specific. When the primary culture rat hepatocytes, labelled with isotopic cromium ( 51 Cr), were treated with the MoAb together with complement, a specific release of 51 Cr from the cells was found shortly after treatment, accompanied with bubbling of the cell membranes, and a significant release of 51 Cr was observed at an MoAb concentration of 15μg/ml or more. Without complement, or with inactivated complement, these reactions were not observed. These facts suggest strongly that the cell surface of the hepatocytes was damaged by the MoAb in the presence of complement. ACTA PATHOL. JPN. 35: 1375–1383, 1985.