Open AccessIMMUNOELECTRON MICROSCOPIC STUDY ON THE CROSS‐REACTIVITY OF ANTIBODIES TO ADULT T‐CELL LEUKEMIA‐ASSOCIATED ANTIGENS IN HUMAN and MONKEY SERA
Author(s) -
Ohtsuki Yuji,
Miyoshi Isao,
Taguchi Hirokuni,
Takeda Isao,
Akagi Tadaatsu
Publication year - 1985
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1985.tb00628.x
Subject(s) - immunoperoxidase , immunoelectron microscopy , virology , antigen , antibody , virus , biology , cell culture , microbiology and biotechnology , monoclonal antibody , immunology , genetics
The cross‐reactivity of antibodies to adult T‐cell leukemia (ATL)‐associated antigens (ATLA) in human and monkey sera was investigated by indirect immunoperoxidase and immunoferritin methods using a human cell line (MT‐2) carrying a type C virus (HTLV), two monkey cell lines (Si‐1 and Si‐3) carrying HTLV, and a monkey cell line (Si‐2) carrying a type C virus isolated from an anti‐ATLA‐positive monkey. Anti‐ATLA‐positive but not‐negative human and monkey sera gave positive immunoperoxidase reaction with all four virus‐positive cell lines when studied by light microscopy. Electron microscopic findings revealed ferritin or peroxidase labeling of virus particles and plasma membranes of these four cell lines with antibody‐positive but not‐negative human and monkey sera. These results clearly indicate the cross‐reactivity of anti‐ATLA antibodies in human and monkey sera at light and electron microscopic levels, and the presence of antigenic determinants common to the surface of type C virus particles of human and monkey origin. ACTA PATHOL. JPN. 35: 863–870, 1985