Open AccessImmunohistological study on malignant fibrous histiocytoma 3
Author(s) -
Inoue Akifumi,
Aozasa Katsuyuki,
Tsujimoto Masahiko,
Tamai Masamitsu,
Chatani Fumio,
Ueno Hiroshi
Publication year - 1984
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1984.tb07604.x
Subject(s) - histiocyte , pathology , histiocytic sarcoma , immunoperoxidase , immunohistochemistry , fibronectin , antibody , medicine , biology , cell , immunology , monoclonal antibody , genetics
Malignant fibrous histiocytoma (MFH) shows a mixed proliferation of both fibroblastic and histiocytic cells. Because of their complex morphologic appearances, the nature of truly neoplastic cells in MFH has been controversial. In the present study, immunoperoxidase method (PAP method) was used to examine the intracytoplasmic lysozyme (LY), alpha‐1‐antitrypsin (A***1AT), fibronectin (FN), and polyvalent immunoglobulin (PI) in the fibroblastic and histiocytic cells. Twenty‐three cases with MFH were histologically divided into three groups; predominantly fibroblastic type (Group I; 5 cases), mixed fibroblastic and histiocytic type (Group II; 15 cases), and almost pure histiocytic type (Group III; 3 cases). Fibroblastic cells showed a strong positive reaction for LY and A1AT, suggesting the histiocytic nature, while the proliferating cells in Group II were more intensely stained by each of the antibodies than in Groups I and III. Enzyme histochemical examinations on fresh materials were available in 3 cases. These findings suggest that proliferating cells in MFH possess a histiocytic nature. Acta pathol. jpn. 34: 759∼765, 1984.