Open AccessUltrastructural Localization Of Endogenous Peroxidase In The Human Thyroid Gland Under Normal And Hyperfunctioning Conditions
Author(s) -
Yamashita Hiroto,
Noguchi Shiro,
Murakami Nobuo,
Moriuchi Akira,
Hodo Masaru,
Yokoyama Shigeo,
Mochizuki Yuichi,
Noguchi Akito,
Nakayama Iwao
Publication year - 1984
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1984.tb07583.x
Subject(s) - endoplasmic reticulum , golgi apparatus , organelle , ultrastructure , thyroid , peroxidase , cytoplasm , chemistry , hydrogen peroxide , thyroid peroxidase , follicular cell , pathology , endocrinology , biochemistry , biology , enzyme , medicine
Ultrastructural localization of human thyroid peroxidase in normal and hyperfunctioning states has been studied. Peroxidase activity was visualized ultrastructurally with a cytochemical reaction using 0.05% diaminobenzidine‐tetrahydrochloride, and hydrogen peroxide with a final concentration of 0. 0025%, 0.005%, or 0.01%, respectively, and 2% osmium tetroxide. Reaction product demonstrating the enzyme was clearly observed in cell organelles with a final concentration of hydrogen peroxide at 0.0025% or 0.005%. In the normal portion of thyroid, follicular cells demonstrated the reaction product, located mainly in rough‐surfaced endoplasmic reticulum and perinuclear cisternae and in addition a small amount of the product was found in Golgi cisternae and small vesicles dispersed throughout the subapical cytoplasm. The reaction product significantly increased in amount in the cell organelles described above and in addition, a characteristic heavy deposition of the product was observed at the external surface of the microvilli in the follicular cells of thyroid obtained from the patients with treated Basedow's disease. ACTA PATHOL. JPN. 34: 553–561, 1984.