Open AccessEFFECTS OF FIBRINOLYTIC TREATMENT ON RABBIT MASUGI NEPHRITIS
Author(s) -
Akiba Tomohide,
Tanaka Kenzo
Publication year - 1983
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1983.tb02126.x
Subject(s) - fibrin , fibrinogen , nephrotoxicity , nephritis , urinary system , chemistry , medicine , pathology , kidney , immunology
We investigated the effects of administration of urokinase (UK) and trans‐4‐aminomethylcyclohexane carboxylic acid (t‐AMCHA) on the development of experimental rabbit Masugi nephritis. Treatment with lOOOU/kg of UK, 5000U/kg of UK and 100 mg/kg of t‐AMCHA twice a day intravenously was initiated on the 7th and continued for 7 consecutive days. Concomitantly with the nephrotoxin injection, 5000U/kg of UK was also given twice a day intravenously for 14 days. The administration of 5000U/kg of UK twice a day for 7 days and 14 days suppressed the increases in BUN values and the decreases in urinary fibrinolytic activity. Fibrin‐flbrinogen degradation products (FDP) were detected more frequently in the urine of rabbits given UK than in the control. Immunofluorescence microscopy showed a decrease in intra‐and extra‐glomerular fibrin deposition, and a decrease in fibrin or fibrinoid deposits in Bowman's space by 5000U/kg of UK was demonstrated electron microscopically. Light microscopy revealed that the accumulation of fibrin in glomeruli, crescent formation, and progressive glomerular disorganization by 5000U/kg of UK were prevented. On the other hand, treatment with t‐AMCHA enhanced the increase in BUN values and there was a decrease in urinary fibrinolytic activity. Dense fibrin deposits in the glomeruli, were observed immunopathologically by treatment with t‐AMCHA and histopathologic changes were found to be even more severe.