Open AccessDOUBLE IMMUNOFLUORESCENCE STUDIES OF IMMUNOGLOBULINS, COMPLEMENT COMPONENTS AND THEIR CONTROL PROTEINS IN PATIENTS WITH IgA NEPHROPATHY
Author(s) -
Tomino Yasuhiko,
Endoh Masayuki,
Nomoto Yasuo,
Sakai Hideto
Publication year - 1982
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1982.tb02046.x
Subject(s) - immunofluorescence , pathology , nephropathy , antibody , complement (music) , immunology , immunoglobulin a , complement system , medicine , indirect immunofluorescence , biology , immunoglobulin g , biochemistry , endocrinology , phenotype , diabetes mellitus , complementation , gene
A study on double immunofluorescent staining of immunoglobulins, complement components, and their control proteins in renal tissues from patients with IgA nephropathy is described. Renal biopsy specimens were obtained from patients with IgA nephropathy. These biopsy specimens were stained with anti sera to human C1q, C4‐binding protein, and β1H globulin by indirect immunofluorescent staining. These samples were then stained with rhodamine‐conjugated anti human IgG, IgM, and IgA. Although C1q and C4‐binding protein do not combine with IgA, they ubiquitously combine with IgG and/or IgM. β1H gobulin also combine with IgG, IgM and/or IgA. It was demonstrated that the complement system activated in IgA nephropathy was via both alternative and classical pathways. The presence of C4‐binding protein in glomeruli appeared to be a more sensitive indicator of classical pathway activation than the presence of C4.