Open AccessEVALUATION OF THE STAINING CONDITIONS OF IMMUNOFLUORESCENCE IN PRIMARY GLOMERULONEPHRITIS
Author(s) -
Tomino Yasuhiko,
Nomoto Yasuo,
Endoh Masayuki,
Sakai Hideto
Publication year - 1981
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1981.tb00988.x
Subject(s) - immunofluorescence , pathology , staining , glomerulonephritis , indirect immunofluorescence , medicine , antibody , immunology , kidney
A study of the evaluation of various staining conditions of immunofluo‐rescence in renal biopsy specimens is described. Renal biopsy specimens were obtained from 52 patients with various types of primary glomerulone‐phritis, and stained with fluorescein‐conjugated anti‐human IgG, IgA, or IgM antisera under various staining conditions. The results indicated that IgM was more prominently stained at 4°C overnight than at 37°C or room temperature by the immunofluorescent staining. Both IgG and IgA did not show any dmerences in the pattern of staining under different conditions. It is suggested that the different staining conditions of immunofluorescence in patients with glomerulonephritis is useful for the evaluation of the classes of immunoglobulins deposited in the glomeruli.