Open AccessIN VITRO TRANSFORMATION OF RAT BRAIN CELLS BY HUMAN ADENOVIRUS TYPE 12
Author(s) -
Mano Shohei
Publication year - 1980
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1980.tb03285.x
Subject(s) - in vitro , pathology , immunofluorescence , biology , histology , microbiology and biotechnology , medicine , immunology , antibody , genetics
In vitro transformation of brain cells cultured from various age rats by human adenovirus type 12 (Ad‐12) was studied. In cultured brain cells, five cell types could be identified light microscopically. In astrocytes, Ac‐2 and Ac‐1, astroprotein was demonstrated by immunofluorescence technique. In the former, intracytoplasmlc microfilaments were recognized by electron microscopy. Undifferentiated cells (Ud), ependymoglioblasts (EGb) and Ac‐1, grew prominently in cultures from young animals or of early passage levels. Ac‐2 and fibroblasts were prominent in cultures from aged animals or of late passage levels. Transformed foci appeared within about 20 days after Inoculation of Ad‐12 and often located in close association with the sheets of Ac‐1. Transformed cells morphologically resembled EGb or transitional cells from EGb to Ac‐1. Transformation rate was high in cultures from young animals or of early passage levels. Subcutaneous transplantation of these cells into newborn rats procued tumors whose histology was similar to Ad‐12‐induced rat brain tumor. T‐Ag were detected both in transformed and transplanted tumor cells. It was concluded, that the target cells of Ad‐12 in vitro correspond with immature brain cells (EGb and/or transitional cells from EGb to Ac‐1 cells).