Open AccessLDH ISOZYME ANALYSES OF ETHYLNITROSOUREA‐INDUCED CENTRAL NERVOUS SYSTEM TUMORS IN RATS
Author(s) -
Yun Kankatsu
Publication year - 1980
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1980.tb01334.x
Subject(s) - oligodendroglioma , ethylnitrosourea , glioma , isozyme , anaplastic astrocytoma , astrocytoma , pathology , meningioma , biology , central nervous system , lactic dehydrogenase , medicine , cancer research , enzyme , endocrinology , biochemistry , gene , mutant
To provide the significance of LDH isozymes in rat CNS tumors, the changes in lactic dehydrogenase isozyme and calculated ratios of H‐ to M‐ subunit were studied by means of polyacrylamide gel enzymoelectrophoresis in tumor extracts from GNS tumors (7 astrocytomas, 4 oligodendrogliomas, 7 mixed gliomas, 6 anaplastic gliomas, 3 glioependymomas, 1 astroblastoma, 11 neurinomas, 8 anaplastic neurinomas and 1 meningioma in Wistar rats which were induced by ethylnitrosourea). The isozyme patterns were compared to those obtained from normal rat CNS tissues. Among the glioma group, oligodendroglioma showed the highest H/M ratio followed by mixed glioma, glioependymoma, astrocytoma, astroblastoma and anaplastic glioma in order of decreasing of H/M ratios. On the other hand, the H/M ratio of neurinoma was significantly higher than that of anaplastic neurinoma. These observation suggested that determination of LDH isozyme patterns could supplement the histological evaluation of brain tumors.