Open AccessMECHANISM OF THE FORMATION OF MEGAMITOCHONDRIA BY COPPER‐CHELATING AGENTS V. FURTHER STUDIES ON ISOLATED MEGAMITOCHONDRIA
Author(s) -
Wakabayashi Takashi,
Asano Masahisa,
Ishikawa Kumiko,
Kishimoto Hidemasa
Publication year - 1978
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1978.tb00533.x
Subject(s) - monoamine oxidase , mitochondrion , chemistry , cytochrome c oxidase , medicine , endocrinology , in vivo , kidney , biochemistry , atpase , biology , enzyme , microbiology and biotechnology
Effect of cuprizone has been studied on some biochemical properties of megamitochondria obtained from the mouse liver. (1) Contents of Ca 2+ , Mg 2+ and Cu 2+ in the blood or the liver homogenates were not altered by cuprizone‐intoxication, whereas those in liver mitochondria were significantly altered: after 3‐4 days’intoxication, content of Ca 2+ was decreased and was remarkably increased after 14‐15 days’intoxication. Content of Mg 2+ behaved contrarily. (2) Both cytochrome oxidase and ATPase activities were unchanged in the liver megamitochondria, but monoamine oxidase (MAO) activity was significantly decreased. Value of I 50 (50% inhibition) for MAO was determined to be 0.33 mM using the control liver mitochondria. Cuprizone had almost no effect on MAO activity of kidney or heart mitochondria both in vivo and in vitro . (3) The amount of lysolecithin was increased in the liver megamitochondria. These results were discussed in the light of membrane fusion phenomenon which plays a key role in the mechanism of megamitochondrial formation.