Open AccessIMMUNOHISTOLOGICAL STUDY ON THE ACTH PRODUCING ISLET CELL CARCINOMA OF THE PANCREAS
Author(s) -
Kawaoi Akira,
Okano Tadao,
Uchida Toshikazu
Publication year - 1977
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1977.tb01875.x
Subject(s) - immunoperoxidase , pathology , antigen , pancreas , argentaffin , biology , medicine , antibody , endocrinology , monoclonal antibody , immunology
ACTH producing cells In islet cell carcinoma associated with Cushing's syndrome were studied by an improved immunoperoxidase as well as immunofluorescence technique. The cells which contained ACTH antigen were distributed throughout the tumor tissue of both primary and metastatic lesions in rather small numbers. In the small cancer nests they were present in an irregular or mosaic pattern and, on the contrary, they tend to line along the margin in the larger nests. The intracytoplasmlc localization of the ACTH antigen was characteristically demonstrated in the intranuclear area of the tumor cells, closely simulating that of the argentaffin granules of the entero‐chromaffin cells. The electron microscopic observation identified membrane enclosed, cored granules,200–300 nm in diameter, present in clusters in the intranuclear area of some tumor cells, which were considered corresponding well to the ACTH positive cells in immune staining.