Open AccessELEKTRONENMIKROSKOPISCHE UND ELEKTRONENMIKROSKOPISCH‐HISTOCHEMISCHE UNTERSUCHUNGEN UBER NEBENNIERENRINDE DER RATTE MIT AMINOGLUTÄTHIMID (ELIPTEN, CIBA) ÜND UBER IHREN REPARATURPROZESS.
Author(s) -
Itoh Gen
Publication year - 1977
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1977.tb01861.x
Subject(s) - mitochondrion , vacuole , digitonin , chemistry , electron microscope , cytoplasm , microbiology and biotechnology , biology , biochemistry , membrane , physics , optics
Amlnoglutethimide blocks the conversion of free cholesterln to D́ 5‐pregnenolone. It's effects on the fasciculata cells were studied electron microscopically in Wistar rats fed with this chemical for a maximum of seven days. The animals surviving after this treatment were also examined to follow the process of restoration from the damage. Four to seven days after the initiation of the feeding, the mitochondria were enlarged, variously deformed and contained intramitochondrial vacuoles. The lipid droplets were increased in size. Three to four days after withdrawal of amlnoglutethimide, so‐called “Intramitochondrial mitochondria” appeared. The picture suggesting division of the mitochondrial matrx was also occasionally encountered. These features suggested formation of new mitochondria from preexisting mitochondria. The mltohchondrla resumed their normal shape and appearance starting about ten days after withdrawal of amlnoglutethimide. The electron microscopic histochemlcal method demonstrated tubular crystals of digitonin‐cholesterid in the intramitochondrial vacuoles as well as in the lipid droplets 4 days after the initiation of the feeding. The withdrawal of amlnoglutethimide was followed with disappearance of these crystals from the sites of deposition.