Open AccessULTRASTRUCTURAL STUDY OF EXPERIMENTAL THROMBOSIS IN PULMONARY MUSCULAR ARTERIES OF RABBITS
Author(s) -
Horie Akio,
Kurozumi Takeshi,
Morimatsu Minora,
Tanaka Kenzo
Publication year - 1974
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1974.tb00816.x
Subject(s) - thrombus , pathology , thrombosis , platelet , endothelium , fibrin , endothelial stem cell , ultrastructure , anatomy , edema , chemistry , medicine , thrombin , in vitro , immunology , biochemistry
In order to clarify the ultrastructural change of initial phase of thrombosis, an autologous clot Injection or one shot of thrombin was performed via an auricular vein of each rabbit. In the groups of clot injection, platelets located between an inserted thrombus and endothelial cells show viscous metamorphosis. Degeneration and disappearance of the endothelial cells are followed by invasion of platelets and dense liquid materials into the subendothelial space and the media of the muscular arteries in both experimental groups. There is no detectable morphological difference between thrombosis in rivo induced by an injection of thrombin and thromboembolism induced by an injection of autologous whole blood clots coagulated in vitro. Phagocytosis in the course of thrombosis is mainly derived from the activity of subendothelial cells and partly from that of endothelial cells. Ultrastructural features of the subendothelial edema are based on the detachment of endothelial cells from the elastic lamina or on the edematous swelling of subendothelial cell cytoplasm. The initial stage of the organization of thrombus, showing smooth muscle cells arranged in the vertical position towards the thrombus, is noted three days after a clot or thrombin administration. Myofilamentous structures with dotted density are detected in the endothelial cells of muscular artery, not only of experimental groups but also of control ones.