Open AccessEXPERIMENTAL AMYLOIDOSIS INDUCED BY IMMUNE COMPLEX: II. AN IMMUNOHISTOLOGICAL OBSERVATION
Author(s) -
Utsuki Yoshio,
Kawaoi Akira,
Hamashima Yoshihiro
Publication year - 1972
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1972.tb00737.x
Subject(s) - spleen , pathogenesis , amyloidosis , immune system , antibody , pathology , antigen , immune complex , antiserum , immunology , medicine , chemistry
Experimental amyloidosis was induced in male albino rabbits and male mice (DD‐strain) with repeated administrations of either antigen‐antibody complexes or antigen and/or Freund's complete adjuvant for a long time. Spleen, lymphnodes, liver and kidneys were studied by light microscopy and fluorescent antibody technique, using anti‐rabbit IgG, anti‐mouse IgG, anti‐HGG or anti‐BSA as antisera. The homogenous substances of liver and spleen were induced in both animals in high frequency. The deposits in the lymphnodes and kidneys were also present in a minority of cases. The peripheral areas or a portion of these substances which were consistent physico‐chemically with para‐amyloid showed weakly positive reaction with fluorescent anti‐HGG or anti‐BSA in the same specimen in spite of the existence of large amounts of antibodies. The tissue toxicity of immune complex may play a significant role in the pathogenesis of amyloidosis in the consideration of the concurrent systemic vascular changes and immune dissociation which suggest the possibility of disturbed immune mechanisms as a common causative background. In the present study using insoluble complexes, some questions still remain to be solved on the pathogenesis of amyloidosis.