Open AccessTHE EFFECT OF MONOCROTALINE, A PYRROLIZIDINE ALKALOID, ON HELA CELLS AND PRIMARY CULTURED CELLS FROM RAT LIVER AND LUNG *
Author(s) -
Umeda Makoto,
Saito Mamoru
Publication year - 1971
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1971.tb00140.x
Subject(s) - hela , vacuole , cytoplasm , vacuolization , parenchyma , pyrrolizidine , lung , pathology , pyrrolizidine alkaloid , biology , mitosis , chemistry , cell , microbiology and biotechnology , biochemistry , medicine , endocrinology , botany
Monocrotaline, a pyrrolizidine alkaloid, produced marked cytoplasmic vacuolization, cellular and nuclear enlargement, and mitotic abnormalities on HeLa cells and primary cultured cells from rat liver and lung. Fat droplets were specifically found in the cytoplasm of liver parenchymal cells. Chromosome preparation of the treated cultures from rat lung revealed marked breaks and fusions. The mechanism of vacuole formation could be ascribed to the basicity of the alkaloid. Severe disorders of chromosomes might correlate to the carcinogenic activity of the compound.