Open AccessELECTRON‐HISTOCHEMICAL STUDIES ON THE LYSOSOME SYSTEM IN ASCITES HEPATOMA AH 13 CELLS
Author(s) -
Sasaki Mitsuo,
Takeuchi Tadao
Publication year - 1969
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1969.tb00089.x
Subject(s) - acid phosphatase , lysosome , golgi apparatus , glycogen , cytoplasm , intracellular , biology , organelle , electron microscope , vacuole , cytochemistry , hepatocyte , biochemistry , microbiology and biotechnology , chemistry , endoplasmic reticulum , enzyme , physics , optics , in vitro
Yoshida ascites hepatoma AH 13 cells were investigated by electron‐microscopy and by electron‐histochemistry combined with the metal precipitation technique for acid phosphatase, especially giving attention to the changes of lysosome system associated with enzyme activity. Abundant deposition of intracellular glycogen appeared not only in form of rosettes, but also more frequently in monoparticulate fashion. Acid phosphatase activity was detectable in vacuolated lysosomes, but their intracellular distribution was a little more random in contrast with that of pericanalicular lysosomes of normal liver cells. In addition, the tumor cell lysosomes appeared slightly irregular in size compared with those of hepatocytes. So‐called myelin figures which were occasionally encountered In the tumor cell cytoplasm were also stainable for acid phosphatase. It is worth mentioning that the Golgi apparatus of AH 13 cells was also acid phosphatase positive contrary to the negative finding in Golgi apparatus of normal liver cells. Glycogen‐containing lysosomes (glycogenosomes) were often found within or in the vicinity of cytoplasmic glycogen areas. The engulfed glycogen particles sometimes lost their granularity and high density, and became amorphous in appearance suggesting their lytic process within the organelle. In quite rare cases, glycogenosome membranes were stained for acid phosphatase. The metabolism in lysosome system of AH 13 cells seems to be considerably different from that of normal liver cells.