Open AccessSTUDIES ON TUMOR INDUCTION IN RATS WITH 7,12–DIMETHYL‐BENZ (a) ANTHRACENE, WITH SPECIAL REFERENCES TO THE MORPHOGENESIS AND FINE‐STRUCTURAL CHANGES IN THE TARGET ORGANS
Author(s) -
Fukunishi Ryo
Publication year - 1968
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1968.tb00037.x
Subject(s) - dmba , pathology , spleen , duct (anatomy) , involution (esoterism) , biology , cancer , chemistry , medicine , carcinogenesis , consciousness , neuroscience
After multiple injections of 7,12–DMBA (25 mg/kg body weight), high yields of malignant tumors were obtained in two different strains of rats (Donryu and ACI). The incidence of mammary cancer was high in female rats of both strains (84.6% in Donryu, 89.5% in AC I). High yields ( 67.5% ) of ear duct cancer were obtained in ACI males, and the incidence of leukemia was highest in Donryu females (46.296). Changes produced with 7, 12–DMBA in several organs were observed by electron microscopy. Acute changes in the organs in which a malignant tumor could be induced appeared irreversible, including steady increase of tonofilaments in the ear duct glandular cell and appearance of numerous plasma cells in the spleen. The changes produced in the liver and kidney were found to be reversible. Morphological discontinuity at the malignant transformation was recognized in the ear duct gland and spleen. Ultrasturctures of induced malignant tumor cells were studied, and mammary cancer was classified into a highly differentiated type and a poorly differentiated type. Classification of organs into three categories, a) DMBA‐responding organ, b) DMBA‐metabolizing and DMBA‐susceptible organ, c) DMBA‐refractory organ, was proposed and each was discussed in detail. ACTA PATH. JAP. 18 : 51–72, 1968.