Open AccessElectron Microscopic and Histochemical Studies on Vacuolar Alteration of Proximal Convoluted Tubules of Rat Kidney after Hypertonic Sucrose Administration
Author(s) -
Hazama Senzo
Publication year - 1964
Publication title -
acta patholigica japonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
ISSN - 0001-6632
DOI - 10.1111/j.1440-1827.1964.tb00687.x
Subject(s) - vacuole , acid phosphatase , cytoplasm , vesicle , organelle , chemistry , lysosome , columnar cell , biophysics , microbiology and biotechnology , biology , anatomy , epithelium , pathology , biochemistry , membrane , medicine , enzyme
Summary Changes of the proximal convoluted tubular epithelium of the rats which were sacrificed instantaneously at 5, 15, 30 minutes, 1, 3, 6, and 8 hours after intraperitoneal injections of 40 to 52 cc per kg of a 50 % aquenous solution of sucrose, were observed by electron microscopic and histochemical methods. The results obtained can be summarized as follows:1) The vacuoles begin to appear 15 minutes after the injection in the apical portion of the tubular cytoplasm and become distinct in 30 minutes. After 6 hours, it is observed in the entire cytoplasm which is filled with large vacuoles. 2) The vacuoles originate from dilatation of simple pinocytotic vesicles which exist physiologically beneath the brush border microvilli. The small vacuoles of 1 to 1.5 microns in diameter reveal heavy acid phosphatase activity and have temporarily a character of so‐called lysosome or cytosome. With the enlargement of vacuoles, the surrounding single limiting membrane reveals discontinuity or rupture at numerous sites. At this stage, the vacuoles aggregate and fuse with each other to form larger ones of 4 to 4.5 microns in diameter and lose their acid phosphatase activity. 3) Vacuolar alteration of the proximal convoluted tubules and the role of lysosome and FCD are discussed.