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Long‐term suppression of methamphetamine‐induced c‐Fos expression in rat striatum by the injection of c‐fos antisense oligodeoxynucleotides absorbed in water‐absorbent polymer
Author(s) -
SEMBA JUN’ICHI,
WAKUTA MAKI,
SUHARA TETSUYA
Publication year - 2004
Publication title -
psychiatry and clinical neurosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.609
H-Index - 74
eISSN - 1440-1819
pISSN - 1323-1316
DOI - 10.1111/j.1440-1819.2004.01296.x
Subject(s) - striatum , methamphetamine , chemistry , messenger rna , microbiology and biotechnology , pharmacology , biology , endocrinology , biochemistry , gene , dopamine
The use of water‐absorbent polymer (WAP) as a hydrogel carrier for the slow delivery of antisense oligodeoxynucleotides (ODN) in the brain, was recently developed. In this experiment, 15‐mer phosphorothioate ODN, complementary to c‐fos gene absorbed in WAP, was injected in the rat striatum. The expression of c‐Fos‐immunoreactivity induced by methamphetamine (6 mg/kg, intraperitoneally) around the injection site was suppressed until 5 days after injection. Using this method, it was observed that unilateral injection with c‐fos antisense ODN into the rat striatum caused robust ipsilateral rotations after methamphetamine challenge 4 days post injection. This method is simple, and the biological and behavioral effects of antisense ODN in WAP can be maintained for several days even after a single injection into the brain.