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Lack of Effect of Haloperidol or Methamphetamine Treatment on the mRNA Levels of Two Dopamine D, Receptor Isoforms in Rat Brain
Author(s) -
Sora Ichiro,
Fujiwara Yutaka,
Tomita Hiroaki,
Ishizu Hideki,
Akiyama Kazufumi,
Otsuki Saburo,
Yamamura Henry I.
Publication year - 1992
Publication title -
psychiatry and clinical neurosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.609
H-Index - 74
eISSN - 1440-1819
pISSN - 1323-1316
DOI - 10.1111/j.1440-1819.1992.tb02868.x
Subject(s) - nucleus accumbens , methamphetamine , haloperidol , ventral tegmental area , substantia nigra , in situ hybridization , pars compacta , medicine , endocrinology , receptor , dopamine , chemistry , gene isoform , messenger rna , dopamine receptor d2 , dopamine receptor , biology , dopaminergic , biochemistry , gene
Abstract: In order to investigate whether changes of the two mRNAs encoding the D 2 receptor isoforms were induced by chronic haloperidol or methamphetamine treatment in rats, we measured the brain mRNA levels using in situ hybridization histochemistry (ISHH). We used two oligonucleotide probes, an “insert” probe to hybridize with the longer D, receptor, D 2(444) , mRNA, and a “spanning” probe to hybridize with the shorter D 2 receptor, D2 (415) , mRNA. Both D 2 mRNAs were detected by ISHH in the caudate putamen, nucleus accumbens, substantia nigra, pars compacta and ventral tegmental men. The distributions and the amounts of the mRNAs for the two D 2 isoforms did not change after chronic administration of haloperidol (1 mg/kg/day for 14 days, ip) or methamphetamine (4 mg/kg/day for 14 days, ip). These results suggest that the changes of D 2 receptor density induced by chronic neuroleptic and psychostimulant treatment are not due primarily to receptor expression.