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Sequence Complexity of Polyadenylate Ribonucleic Acid in El Mouse Brain
Author(s) -
Hirayama Eiichi,
Yamagami Sakae,
Onishi Hiroshi,
Chikami Takashi,
Mori Koichi,
Kawakita Yukio
Publication year - 1986
Publication title -
psychiatry and clinical neurosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.609
H-Index - 74
eISSN - 1440-1819
pISSN - 1323-1316
DOI - 10.1111/j.1440-1819.1986.tb03162.x
Subject(s) - messenger rna , rna , microbiology and biotechnology , precursor mrna , biology , nucleic acid thermodynamics , dna , genetics , gene , rna splicing
To elucidate the sequence complexity of polyadenylate messenger RNA [poly(A+)mRNA] regarding the seizure‐susceptible E1 mouse brain, the proportion of complexity was measured by saturation hybridization using a single‐copy DNA (scDNA) with excess RNA. At saturation, 3.81, 3.85 and 4.00% of scDNA hybridized with polysomal poly (A)mRNAs of El(+), EI(O) which had not been convulsed, and ddY (nonsusceptible) mice, respectively. The additive hybridization of a mixture of El(+) and ddY mouse mRNAs was 4.04%. The results suggest that there are considerable overlapping sets within the poly(A)mRNA sequences between the ddY and El mice, but the complexity of ddY mice mRNA is slightly larger than that of the El mice, whereas poly(A) heterogeneous nuclear RNA (hnRNA) hybridized with about 18% of scDNA in three strains of mice.