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IMMUNOHISTOLOGICAL STUDIES OF EXPERIMENTAL MYOSITIS IN RELATION TO HUMAN POLYMYOSITIS WITH SPECIAL REFERENCE TO PASSIVE TRANSFER AND FLUORESCENT ANTIBODY TECHNIQUE
Author(s) -
Takayanagi Tetsuya
Publication year - 1967
Publication title -
psychiatry and clinical neurosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.609
H-Index - 74
eISSN - 1440-1819
pISSN - 1323-1316
DOI - 10.1111/j.1440-1819.1967.tb00081.x
Subject(s) - myositis , polymyositis , pathology , medicine , ouchterlony double immunodiffusion , spleen , antibody , autoimmune disease , immunology , chemistry , antiserum
S ummary Immunohistological investigation of experimental myositis was performed. This was produced in guinea‐pigs given 10 or 20 injections of 20% homologous muscle homogenate mixed with Freund's complete adjuvant at weekly intervals. Ouchterlony's agglutination test showed a negative reaction between the muscle homogenate and the animal serum. On the other hand strong fluorescence was observed in some muscle fibers. Intraperitoneal injection of spleen and lymph‐gland cells, removed from six donor guinea‐pigs with experimental myositis, produced muscle changes 2–7 days after the transfer similar to those of donor animals. This occurred all six recipient guinea‐pigs, which had been previously treated with 400r. whole body irradiation. The number of cells transferred ranged from 1.1 × 10 8 to 1.3 × 10 9 . Capability of passive transfer and delayed type skin reaction in experimental myositis as compared with polymyositis as autoimmune disease were discussed. Clinical findings of polymyositis as well as similarity to experimental myositis led to considering an autoimmune disease. However, there remain many problems to be solved from the immunological point of view.