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METABOLISM OF STIMULATED SLICES FROM CEREBRAL CORTEX
Author(s) -
Takumi Akira
Publication year - 1962
Publication title -
psychiatry and clinical neurosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.609
H-Index - 74
eISSN - 1440-1819
pISSN - 1323-1316
DOI - 10.1111/j.1440-1819.1962.tb01210.x
Subject(s) - stimulation , incubation , oxygen , substrate (aquarium) , metabolism , chemistry , malonate , cerebral cortex , lactic acid , biochemistry , biophysics , medicine , endocrinology , biology , bacteria , ecology , genetics , organic chemistry
Summary A study on the relation between the metabolic response to electrical stimulation and substrates, using slices of cerebral cortex of mature rats led to the following conclusions: 1. When glucose was used as substrate, electrical stimulation caused about 50 % increase of oxygen consumption and about 110% increase of lactic acid formation in the slices. Inorganic phosphates also increased thereby. The rise of respiratory activity was markedly inhibited by malonate. 2. Without use of substrate, electrical stimulation, when applied in the early stage of incubation, caused increase of oxygen consumption in the specimens. 3. When succinate acted as substrate, the oxygen consumption again rose by electrical stimulation, even when applied first after 30 min. of incubation. unlike in the test without substrate. 4. When citrate was used as substrate, application of electrical stimulation after 30 min. of incubation was followed by marked increase of oxygen consumption. The above results were adduced in discussion of the stimulated metabolism under electrical stimulation and other stimulation systems including the so‐called potassium effect in comparison.