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ION EXCHANGE CHROMATOGRAPHIC STUDIES ON ACID‐SOLUBLE BRAIN NUCLEOTIDES AND INCORPORATION OF P 32 INTO THEM
Author(s) -
Ishikawa Akio
Publication year - 1961
Publication title -
psychiatry and clinical neurosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.609
H-Index - 74
eISSN - 1440-1819
pISSN - 1323-1316
DOI - 10.1111/j.1440-1819.1961.tb00640.x
Subject(s) - nucleotide , adenine nucleotide , chemistry , chromatography , polyphosphate , ion chromatography , fractionation , ion exchange resin , phosphorus , metabolism , biochemistry , phosphate , organic chemistry , gene
Summary By the modified method of ion exchange resin chromatography of Hurlbert et al, which is designed to perform fractionation in a short time with relatively simple instruments, the brain acid‐soluble nucleotide of albino rats were seperated and measured. Under the condition of freezing animals with dropping of liquid air, adenine nucleotide accounted for about 70 % of all nucleotide, and ATP, ADP, AMP were each 0.55, 0.37, 0.36/xM per wet gram of the brain, ATP/ADP being 1.49. nucleotide polyphosphate, especially ATP, were decomposed soon after the death of animals to get vanished at about 30 minutes, while the decrease of ADP was slower than that of ATP. As the result of investigation on the cerebral phosphorus metabolism utilizing P 32 in ion exchange resin chromatography, it proved that P 32 was rapidly incorporated into the labile‐P such as ATP and ADP in case of subaraehnoideal P 32 injection with about the same speed as in case of the liver. The main reason why the turn‐over from inorganic phosphorus to ATP and ADP was delayed apparently in the case of intraperitoneal P 32 injection consists presumably in the unavoidable blood interfusion into the brain in cases of freezing and extraction.