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L‐ASPARTIC ACID FORMATION PROM N‐ACETYL‐L‐ASPARTIC ACID IN THE BRAIN
Author(s) -
Mori By Akitane
Publication year - 1961
Publication title -
psychiatry and clinical neurosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.609
H-Index - 74
eISSN - 1440-1819
pISSN - 1323-1316
DOI - 10.1111/j.1440-1819.1961.tb00629.x
Subject(s) - aspartic acid , centrifugation , chemistry , spleen , hamster , kidney , biochemistry , specific activity , chromatography , microbiology and biotechnology , biology , enzyme , amino acid , endocrinology , immunology
Summary 1. Radioactive L‐aspartic acid was produced by incubating C i*‐N‐acetyl‐L‐aspartic acid with a mouse brain homogenate. 2. Glutathione and Co ++ must co‐exist to activate this reaction. However, the reaction could be performed more or less by using Fe ++ , Cu ++ , Ca + + , and Zn* + instead of Co* H . 3. The optimal pH of the reaction was 7.0. 4. The reaction took place not only in the brain of the mouse but also in the human, cat, hamster and rabbit brains, and there was not any significant difference among the activities in these different species. 5. The reaction was performed not only in the brain, but in the kidney, the liver and the spleen; the liver and spleen showing more activity than the brain, and the kidney at the most. 6. The investigation of the activities by the fractionation of the brain homogenate by ultra‐centrifugation revealed that the reaction is remarkable in the supernatant of 18,00OXg centrifugation.