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Isolation of functional dendritic cells from murine kidneys for immunological characterization
Author(s) -
TETERIS SIMON ALEXANDER,
HOCHHEISER KATHARINA,
KURTS CHRISTIAN
Publication year - 2012
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/j.1440-1797.2012.01581.x
Subject(s) - dendritic cell , centrifugation , microbiology and biotechnology , isolation (microbiology) , kidney , differential centrifugation , follicular dendritic cells , parenchyma , population , biology , immunology , medicine , immune system , antigen presenting cell , pathology , t cell , biochemistry , bioinformatics , genetics , environmental health
Aim: The kidney is a complex organ, requiring the contributions of multiple cell types to perform its various functions. Within this system the dendritic cell has been demonstrated to play a key role in maintaining the immunological balance of the kidney. In this methods paper we aim to identify the best method for isolating murine renal dendritic cells. Methods: The efficiency of isolating dendritic cells from enzymatically digested renal parenchyma by density centrifugation, MACS and FACS was compared. Results: Density centrifugation enriched dendritic cells by only approximately two fold. However, MACS and FACS resulted in a much higher purity (80% versus 95% respectively). Conclusions: Although FACS gave the highest purity, MACS is the optimal method for isolating dendritic cells given cost and time factors. Isolation of a homogeneous population of renal dendritic cells will enable the molecular and functional dissection of these cells in both homeostasis and disease models.