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Effects of recombinant human endostatin on peritoneal angiogenesis in peritoneal dialysis rats
Author(s) -
ZHAO ZHANZHENG,
CAO YING,
LIU ZHANGSUO,
XIAO JING,
TANG LIN,
WANG PEI,
LIANG XIANHUI
Publication year - 2011
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/j.1440-1797.2011.01465.x
Subject(s) - endostatin , medicine , angiogenesis , vascular endothelial growth factor , recombinant dna , basic fibroblast growth factor , immunohistochemistry , peritoneal dialysis , endocrinology , messenger rna , neovascularization , peritoneum , downregulation and upregulation , andrology , pathology , growth factor , vegf receptors , receptor , biology , biochemistry , gene
Aim: To investigate the effects of recombinant human endostatin (Endostar) on peritoneum angiogenesis in a model of dialysate exposure in rats. Methods: Forty male Sprague–Dawley rats were randomized to five groups: normal (group 1); uraemia (group 2); 4.25% peritoneal dialysate (PD) uraemic (group 3); uraemia + PD + recombinant human endostatin 10 mg/kg PD (group 4); and uraemia + PD + recombinant human endostatin 40 mg/kg PD (group 5). The uraemic rats model was established by 5/6 nephrectomy. Endostatin was administrated by s.c. injection every other day, over 28 days. After 28 days of PD fluid exposure, immunohistochemistry and reverse transcript polymerase chain reaction were used to detect protein and mRNA expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in each group. Microvessel density (MVD) was measured by immunohistochemistry. Results: Compared with group 1, the mRNA and protein expressions of VEGF and bFGF were significantly upregulated in groups 2 and 3 ( P < 0.05). Compared with group 3, the mRNA and protein expressions of VEGF and bFGF were significantly downregulated in groups 4 and 5 ( P < 0.05). Compared with group 4, the mRNA and protein expressions of VEGF and bFGF were significantly downregulated in group 5 ( P < 0.05). Compared with group 1, MVD was significantly upregulated in groups 2 and 3 ( P < 0.05). Compared with group 3, MVD was significantly downregulated in groups 4 and 5 ( P < 0.05). Conclusion: Endostar can effectively inhibit rat peritoneum neoangiogenesis and the effect was dose‐dependent.