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Renal artery injection for delivery of biological materials to the glomerulus (Methods in Renal Research Paper)
Author(s) -
TAKABATAKE YOSHITSUGU,
ISAKA YOSHITAKA,
IMAI ENYU
Publication year - 2008
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/j.1440-1797.2007.00907.x
Subject(s) - glomerulus , electroporation , green fluorescent protein , gene silencing , renal artery , medicine , small interfering rna , gene delivery , perfusion , microbiology and biotechnology , transfection , renal glomerulus , transgene , kidney , gene , biology , biochemistry , glomerulonephritis
SUMMARY: Aim: We investigated whether injection of synthetic small interfering siRNAs via renal artery followed by electroporation could be effective in silencing specific genes in glomerulus. Methods: Solution of siRNA targeting enhanced green fluorescent protein (EGFP) was injected via the renal artery of EGFP‐transgenic rats followed by delivery of electric pulses. Results: The delivery of siRNA reduced endogenous EGFP expression, mainly in glomerular mesangial cells. Conclusion: Electroporation‐mediated gene transfer system via renal artery in rats is very useful for the delivery of a range of different materials including cells, viruses and proteins. Here, we provide a step‐by‐step description of this perfusion method and a discussion of the key points for the success of the technique.