Premium
Metalloproteinase activity is present in rat urine and derived from the renal cortex
Author(s) -
SATO Yuji,
FUJIMOTO Shouichi,
HAMAI Keiko,
ETO Tanenao
Publication year - 1997
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/j.1440-1797.1997.tb00272.x
Subject(s) - urine , renal cortex , urinary system , kidney , endocrinology , cortex (anatomy) , matrix metalloproteinase , medicine , metalloproteinase , chemistry , biology , neuroscience
Summary: Matrix metalloproteinases (MP) are important candidates for the degradation of extracellular matrix, but the role of MP in the diseased kidney remains poorly understood. to examine the significance of urinary MP, we first investigated the characteristics of MP in normal rat urine and renal cortex, and then evaluated the urinary MP activity in anti‐thymocyte induced glomerulonephritis (Thy.1 GN). Metalloproteinase activity was measured as the EDTA‐inhibitable degradation of [ 3 H] gelatin. the enzyme was purified from urine and the renal cortex homogenate in normal Wistar rats by using several chromatographic and gel filtration methods. Both materials contained the identical molecular weight (Mr 126 kDa by gel permeation method) of gelatin‐degrading enzymes, the activity of which was inhibited by metal chelating agents and reactivated by ZnC1 2 but not by other proteinase inhibitors. Thy.1 GN was induced by intravenous injection of rabbit anti‐thymocyte serum into rats, and daily urine was collected at sequential time points. Urinary MP activity was markedly reduced soon after the serum injection, and returned to the control level in 9 weeks. Conversely, urinary MP‐inhibitor activity (Mr 30 kDa), determined as inhibiting activity against MP derived from renal cortex, showed serial changes strikingly reflected as urinary MP activity. These findings suggested that rat urine contained the MP which seemed to be derived from the renal cortex, and the urinary MP activity was decreased in Thy.1 GN model, probably due to the presence of MP‐inhibitor. As urinary MP is likely to reflect intra‐renal MP, the evaluation of urinary MP may be useful to search metabolic alteration of extracellular matrix in the diseased kidney.