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Enhancement of chronic cyclosporine nephrotoxicity by sodium depletion in an experimental mouse model
Author(s) -
ANDOH Takeshi F,
LAM Tri T,
LINDSLEY Jessie,
ALPERS Chaiues E,
BENNETI William M
Publication year - 1997
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/j.1440-1797.1997.tb00271.x
Subject(s) - nephrotoxicity , medicine , blood urea nitrogen , creatinine , dose , pharmacology , placebo , endocrinology , fibrosis , sodium , sodium salicylate , nephrocalcinosis , kidney , pathology , chemistry , alternative medicine , organic chemistry
Summary: The major adverse reaction to cyclosporine A (CsA) is chronic nephrotoxicity. to elucidate the pathogenesis of CsA nephrotoxicity and the potential role of the renin‐angiotensin system (RAS) in mediating this toxicity, a reliable mouse model would enable us to take advantage of various probes, and gene knockout animals available in this species, but not in the rat, would be extremely useful. Using the manoeuvre of sodium depletion and activation of RAS, an experimental model in the mouse has been developed to reproduce structural and functional characteristics of chronic CsA nephrotoxicity in humans. Mice were treated with CsA subcutaneously at dosages of 10 mg/kg per day and 30 mg/kg per day or olive oil as a placebo on a low sodium diet (LS) (0.01% sodium) for 28 days. Comparisons were made with mice treated with CsA at 100 mg/kg per day on a normal sodium diet (NS; 0.4% sodium) for 56 days. Cyclosporine A treatment induced significant increases in serum creatinine and blood urea nitrogen (BUN) and a decrease in creatinine clearance in mice on LS ( P <0.05 vs placebo). Sodium‐depleted animals on CsA 30mg/kg per day treatment developed significant histological lesions in the kidneys consisting of tubulointerstitial fibrosis, arteriolopathy, nephrocalcinosis, and tubular injury after 28 days ( P <0.001 vs placebo). By contrast, CsA treatment in mice on NS did not induce functional and structural abnormalities in the kidneys even up to 56 days at a higher dose. This mouse model can be useful to gain further insights into the pathogenetic mechanisms of chronic CsA nephropathy.