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Modification of mesangial cell function by ambient chloride is absent in Dahl salt sensitive rat
Author(s) -
OHARA Mamiko,
OKUDA Toshihiro,
INISHI Yoji,
KUROKAWA Kiyoshi
Publication year - 1997
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/j.1440-1797.1997.tb00270.x
Subject(s) - mesangial cell , endocrinology , medicine , prostaglandin e2 , vasopressin , cell , chemistry , kidney , biochemistry
Summary: We previously reported that the normally present modification of mesangial cell function by ambient chloride concentration ([Cl]) and insulin was lacking in mesangial cells obtained from spontaneously hypertensive rats (SHR). This aberrant modification of the mesangial cell might be related to the development of hypertension. In this study, we examined if a similar aberrant modification of mesangial cell function by [Cl] and insulin was present in Dahl salt sensitive (DS) rats, which develop hypertension in a NaCl dependent manner. Similar to our previous reports on mesangial cells from normal rats, pre‐treatment with low [Cl] apparently attenuated Ca responses induced by angiotensin II and vasopressin in mesangial cells cultured from Dahl salt resistant (DR) rats. In contrast, this attenuation of vasoactive agents‐induced Ca signal was absent in the DS rat derived‐mesangial cells. Also, the enhancement of prostaglandin E2 production caused by decrease in [Cl] was not observed in the DS rat mesangial cells, while prostaglandin E2 production by DR rat mesangial cell was stimulated when [Cl] was decreased. In contrast, vasopressin‐induced Ca signal by insulin was attenuated in both DR and DS rat mesangial cells. Thus, in DS rat mesangial cells, modification of mesangial cell function by [Cl] was absent, but it was different from SHR mesangial cells because insulin was maintained.