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Activation of mRNA expression of collagen, collagenase and its inhibitor on renal biopsy specimens in patients with IgA nephropathy
Author(s) -
SAKAI Hideto,
JINDE Kiichiro,
SAOTOME Noboru,
SUNG Wei,
YAGAME Mitsunori,
NOMOTO Yasuo,
MIYAZAKI Masanobu,
HARADA Takashi
Publication year - 1997
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/j.1440-1797.1997.tb00196.x
Subject(s) - nephropathy , collagenase , medicine , diabetic nephropathy , glomerulonephritis , messenger rna , biopsy , pathology , tissue inhibitor of metalloproteinase , renal biopsy , in situ hybridization , endocrinology , kidney , matrix metalloproteinase , diabetes mellitus , biology , enzyme , biochemistry , gene
Summary: In situ hybridization of mRNA for collagen IV, collagen VI, stromelysin (MMP‐3) and TIMP1 was examined in renal biopsy specimens from patients with IgA nephropathy (IgAN) or diabetic nephropathy with various degrees of tissue damage. The majority of cells in the glomeruli expressed these mRNA almost simultaneously, but a few cells demonstrated positive expression for only one of these probes. There was a parallel relationship between the degree of tissue damage and that of mRNA expressions of these probes in patients with IgAN, while patients with diabetic nephropathy showed a reverse relationship between these two parameters. It is concluded that patients with mesangial proliferative glomerulonephritis expressed mRNA for collagen collagenase and its inhibitor in the glomeruli in parallel with the progress of tissue damage. In contrast, glomerular samples from patients with diabetic nephropathy showed that there was an inverse relationship between tissue damage and expression of mRNA. It is concluded that expression of collagen, collagenase and its inhibitor parallels the progression of glomerular changes in IgAN, but such parallel expression was not observed in patients with diabetic nephropathy.