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Acute infusion of amphotericin B: Proximal tubular effects
Author(s) -
POLLOCK Carol A,
DYNE Marlen,
FIELD Michael J,
MOORE Noriah SALIPAN,
REDDY Sri,
COCKAYNE David,
GYÖRY ÁKos Z
Publication year - 1996
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/j.1440-1797.1996.tb00120.x
Subject(s) - amphotericin b , intracellular , medicine , renal function , endocrinology , saline , pharmacology , chemistry , biochemistry , antifungal , dermatology
Summary: Electron microprobe analysis (EMPX) was employed to determine the alterations in intracellular electrolyte concentrations in proximal tubular cells resulting from acute treatment with amphotericin B. Concurrent clearance data confirmed a drop in glomerular filtration rate (GFR) in the rats treated with amphotericin B infusion (1 mg/kg per h) compared to control animals infused with saline (0.69 ± 0.11 vs 1.27 ± 0.08 mL/min per 100 g; P < 0.001). Measurement of intracellular electrolytes in proximal tubular cells in mmol/kg wet weight by EMPX demonstrated no difference in Na (16.2 ± 0.6 vs 17.2 ± 0.5) or K (129.7 ± 2.1 vs 131.3 ± 2.0). Intracellular Rb accumulation following acute infusion was significantly reduced in the amphotericin B treated animals (3.9 ± 0.4 vs 6.6 ± 0.4; P < 0.0001), suggestive of a reduction in basolateral Na‐K ATPase activity. These results do not support the tenet that amphotericin B causes a generalized increase in epithelial cell membrane ionic permeability, nor direct tubular toxicity in the doses and time frame studied. Rather, they suggest that a primary reduction in GFR results acutely in a load‐dependent decrease in proximal tubular Na transport.