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Modulators of growth in primary culture of rat proximal tubular cells
Author(s) -
NOBES Michael S,
POLLOCK Carol A,
HENG Pan T,
FIELD Michael J
Publication year - 1995
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/j.1440-1797.1995.tb00011.x
Subject(s) - medicine , endocrinology , muscle hypertrophy , thymidine , epidermal growth factor , hyperplasia , cell growth , nephrectomy , growth factor , renal hypertrophy , kidney , cell culture , dna synthesis , transforming growth factor , stimulation , cell , somatomedin , biology , in vitro , receptor , biochemistry , diabetic nephropathy , genetics
Summary: The effects of known growth factors and circulating serum factors in animals following unilateral nephrectomy on cellular hypertrophy and hyperplasia were studied in primary culture of rat proximal tubular cells. the cultured cells were derived from normal rat kidney, as well as from rat kidneys which had undergone compensatory renal hypertrophy. Cellular thymidine incorporation was stimulated with insulin‐like growth factor 1 (IGF‐1; P <0.001), epidermal growth factor (EGF; P <0.01) and additively by the combination of IGF‐1+EGF ( P <0.0001). Transforming growth factor β 1 (TGF β 1 ) potently inhibited thymidine incorporation in the presence or absence of these growth factors. Cellular protein content was increased in the presence of IGF‐1 ( P <0.01). the addition of 10% serum harvested from sham operated (Sx) animals to cell cultures derived from Sx animals significantly increased cell protein content ( P <0.02), and stimulated cellular thymidine incorporation ( P <0.005). the addition of 10% serum harvested from unilaterally nephrectomized (Nx) animals further enhanced the growth response. In cell cultures derived from Nx rats, a further marked stimulation of thymidine incorporation and cellular protein was observed in the presence of both Sx and Nx serum, indicating an increased sensitivity of these cells to serum‐derived factors. In summary, the growth factors tested have direct effects in regulating rat renal tubular cell growth. Circulating factors stimulating proximal tubular cell growth are present in animals which have undergone nephrectomy. Renal tubular cells derived from animals with compensatory renal hypertrophy are sensitized to the effects of the manipulations which enhance proximal tubular cell growth.