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Pathological study of pseudohypertrophy of the inferior olivary nucleus
Author(s) -
Ogawa Katsuhiko,
Mizutani Tomohiko,
Uehara Kenji,
Minami Masayuki,
Suzuki Yoshio,
Uchihara Toshiki
Publication year - 2010
Publication title -
neuropathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 61
eISSN - 1440-1789
pISSN - 0919-6544
DOI - 10.1111/j.1440-1789.2009.01033.x
Subject(s) - synaptophysin , inferior olivary nucleus , immunohistochemistry , neurite , immunostaining , nucleus , kdel , cytoplasm , chemistry , pathology , microbiology and biotechnology , biology , medicine , golgi apparatus , biochemistry , endoplasmic reticulum , in vitro
There have been only a few reports about the immunohistochemical study of pseudohypertrophy of the inferior olivary nucleus (PH‐IO). We therefore performed the detailed immunohistochemical study of 10 PH‐IOs in 8 patients to clarify the mechanism of neuronal degeneration and its related phenomenon of PH‐IO. We used various antibodies to αB‐crystallin (αBC), synaptophysin (SYP), microtubule‐associated protein 2 (MAP2), Lys‐Asp‐Glu‐Leu (KDEL) receptors, heat shock protein (HSP) 27 as well as SMI‐31. We found αBC‐positive neurons on the ipsilateral side of 10 PH‐IOs. SMI‐31‐positive neurons were also observed in 6 PH‐IOs. Confocal laser microscopy showed co‐localization of αBC and SMI‐31 in some neurons. However, there were no HSP27‐positive neurons or astrocytes in any of the 10 PH‐IOs. MAP2 immunostaining showed MAP2‐positive hypertrophic thick neurites around hypertrophic neurons on the ipsilateral side of 7 PH‐IOs and demonstrated “glomeruloid structures” in 3 PH‐IOs. In addition, fine granular SYP‐immunoreactivity was decreased in the neuropils on the ipsilateral side of all 10 PH‐IOs. SYP‐immunoreactive dots were scattered in the neuropils and on the neuronal cell bodies on the side of 7 PH‐IOs, and the aggregation of SYP‐immunoreactive dots scattered in the neuropils was shown in 3 PH‐IOs. Double‐immunostainings using anti‐MAP2 and anti‐SYP antibodies demonstrated frequent SYP‐immunoreactive dots along the MAP2‐positive hypertrophic thick neurites and their cell bodies. Periphery‐stained KDEL‐positive neurons were also found on the side of 7 PH‐IOs. We showed that the change of the distribution of presynaptic terminals correlated well to the hypertrophic thick neurites in PH‐IO. Our immuohistochemical stainings demonstrated various changes which occurred to the neurons in PH‐IO, and their neurites and presynaptic terminals. We considered that αBC was expressed in the neurons in PH‐IO, induced by cellular stress. Such a detailed immunohistochemical investigation has not been reported previously.