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Quantitative mRNA expression analysis of neurotrophin‐receptor TrkC and oncogene c‐MYC from formalin‐fixed, paraffin‐embedded primitive neuroectodermal tumor samples
Author(s) -
Kunz Franzisca,
Shalaby Tarek,
Lang Doris,
Von Büren André,
Hainfellner Johannes A.,
Slavc Irene,
Tabatabai Ghazaleh,
Grotzer Michael A.
Publication year - 2006
Publication title -
neuropathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 61
eISSN - 1440-1789
pISSN - 0919-6544
DOI - 10.1111/j.1440-1789.2006.00694.x
Subject(s) - tropomyosin receptor kinase c , neurotrophin 3 , biology , oncogene , messenger rna , pathology , rna , cancer research , immunohistochemistry , microbiology and biotechnology , receptor , medicine , cancer , gene , brain derived neurotrophic factor , genetics , growth factor , cell cycle , neurotrophic factors , platelet derived growth factor receptor
Most recent studies analyzing candidate biological prognostic factors (including neurotrophin receptor TrkC and proto‐oncogene c‐MYC) in childhood primitive neuroectodermal brain tumors (PNET) are limited by small patient numbers due to dependence on fresh‐frozen tumor material. In contrast, large archives of formalin‐fixed, paraffin‐embedded PNET samples exist from homogeneously treated patients. The ability of real‐time RT‐PCR to assay very small mRNA fragments makes this assay amenable to studies where the RNA is moderately or even highly degraded. We have optimized RNA isolation from archive PNET samples and found that TrkC and c‐MYC mRNA measurements significantly correlated with those obtained from matching fresh‐frozen tissues. Exploitation of already existing archives of formalin‐fixed paraffin‐embedded PNET samples may accelerate the building of better stratification systems for PNET patients.