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Lectin cytochemistry of rat cerebral and cerebellar neuronal cells
Author(s) -
Kawai Kensuke,
Ishizu Hideki,
Kuroda Shigetoshi
Publication year - 2001
Publication title -
neuropathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 61
eISSN - 1440-1789
pISSN - 0919-6544
DOI - 10.1111/j.1440-1789.2001.00362.x
Subject(s) - lectin , cytochemistry , glycoconjugate , cerebellum , griffonia simplicifolia , central nervous system , deep cerebellar nuclei , biology , chemistry , microbiology and biotechnology , biochemistry , neuroscience , cerebellar cortex , enzyme
Lectin cytochemistry of normal rat central nervous system (CNS) was performed on formalin‐fixed, paraffin‐embedded sections by using 14 lectins and light and electron microscopy. Hippocampal neurons reacted positively with Griffonia ( Bandeiraea ) simplicifolia lectin I (GSL‐I), Griffonia ( Bandeiraea ) simplicifolia lectin II (GSL‐II) and Vicia villosa agglutinin (VVA); neurons in the cornu ammonis (CA) 2 region reacted positively with GSL‐I and GSL‐II, and neurons in the CA2 and CA3 regions reacted with VVA. Cortical neurons reacted positively with GSL‐I and VVA. Cerebellar Purkinje cells and granule cells were reactive with Phaseolus vulgaris leukoagglutinin (PHA‐L). Cerebellar nucleus neurons were also reactive with PHA‐L and VVA. Reactivity with GSL‐I, GSL‐II, VVA and PHA‐L was observed only on the neuronal cell surface. The present findings demonstrate the differences between cerebral and cerebellar neurons in reacting with glycoconjugates.