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Ultracytochemical demonstration of the polarity of Ca 2+ ‐ATPase activity in microglia
Author(s) -
Fukai Kosuke,
Kawai Kensuke,
Ishizu Hideki,
Tanabe Yasuyuki,
Komoto Yasunobu,
Kuroda Shigetoshi
Publication year - 1998
Publication title -
neuropathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 61
eISSN - 1440-1789
pISSN - 0919-6544
DOI - 10.1111/j.1440-1789.1998.tb00116.x
Subject(s) - microglia , basal lamina , polarity (international relations) , chemistry , atpase , biophysics , neuroglia , microbiology and biotechnology , biology , ultrastructure , biochemistry , central nervous system , anatomy , neuroscience , inflammation , cell , enzyme , immunology
In order to investigate plasma membrane Ca 2+ ‐ATPase (Ca 2+ ‐ATPase) activity in three subsets of microglia/macrophages (microglia in perineuronal regions (perineuronal microglia)), microglia in contact with the parenchymal side of the vascular basal lamina (juxtavascular microglia) and perivascular cells within the basal lamina), we performed an ultracytochemical study using a lead salt technique in rat cerebral cortices. In 60.6% of perineuronal microglia, the cytochemical reaction (CR) for Ca 2+ ‐ATPase on the plasma membrane (PM) in the vicinity of neurons was stronger than the CR on the other side of the PM, and deposits of the reaction product (RP) for Ca 2+ ‐ATPase had accumulated, reflecting the polarity of Ca 2+ ‐ATPase activity. In contrast, in all of juxtavascular microglia and perivascular cells, deposits of the RP were distributed evenly along the PM without polarity. These results suggest that the polarity of the Ca 2+ ‐ATPase activity manifests certain functional interactions between perineuronal microglia and neurons.