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Ganglioside GD3 of cerebral neurons and Purkinje cells in aged human brains
Author(s) -
Kawai Kensuke,
Watarai Shinobu,
Ishizu Ideki,
Fukai Kosuke,
Yasuda Tatsuji,
Kuroda Shigetoshi
Publication year - 1997
Publication title -
neuropathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 61
eISSN - 1440-1789
pISSN - 0919-6544
DOI - 10.1111/j.1440-1789.1997.tb00054.x
Subject(s) - lipofuscin , dentate nucleus , cerebellum , pathology , ganglioside , amyotrophic lateral sclerosis , dentate gyrus , progressive supranuclear palsy , biology , senile plaques , neuroscience , atrophy , alzheimer's disease , chemistry , medicine , central nervous system , biochemistry , disease
Immunocytochemical staining for ganglioside GD3 (II 3α (NeuAcα2–8NeuAc)‐LacCer, GD3) in neuronal cells of the cerebral cortices (cerebral neurons), and cerebellar dentate nucleus (dentate neurons) and Purkinje cells, in human autopsy cases of progressive supranuclear palsy, senile dementia of the Alzheimer type, Pick's disease, amyotrophic lateral sclerosis and olivopontocerebellar atrophy (OPCA) was undertaken using mouse IgM anti‐GD3 monoclonal antibody. Cerebral neurons and dentate neurons were constantly GD3‐immunoreactive and immunoreactivity was observed in the cytoplasm. The peroxidase reaction product for GD3 (RP) in cerebral and dentate neurons was granular in appearance. It appeared that RP was associated with lipofuscin granules. However, immunoreactivity of Purkinje cells varied among cases, and the RP was slightly granular even when they were positive. This study suggests that lipofuscin granules contributed to the neuronal immunoreactivity of GD3 in aged human brains.