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Ultrastructural localization of heparan sulfate‐like immunoreactivity in spinal spheroids of motor neuron disease
Author(s) -
Kurita Keiji,
Kato Takeo,
Wada Manabu,
Kawanami Toru,
Sasaki Hideo
Publication year - 1997
Publication title -
neuropathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 61
eISSN - 1440-1789
pISSN - 0919-6544
DOI - 10.1111/j.1440-1789.1997.tb00005.x
Subject(s) - neurofilament , heparan sulfate , immunoperoxidase , axon , immunogold labelling , spinal cord , motor neuron , pathology , keratan sulfate , ultrastructure , microbiology and biotechnology , immunocytochemistry , chemistry , glycosaminoglycan , biology , immunohistochemistry , anatomy , proteoglycan , immunology , medicine , antibody , neuroscience , extracellular matrix , monoclonal antibody
It has recently been reported that heparan sulfate (HS)‐like immunoreactivity exists in spheroids of the spinal cord in motor neuron disease (MND). However, the association of the HS‐like immunoreactivity with neurofilaments, which are the main structure in spinal spheroids of MND, remains to be clarified. The aim of the present study is to elucidate the ultrastructural localization of HS‐like immunoreactivity. The spinal cords from five autopsy cases of sporadic MND and two control cases were immunocytochemically investigated with an antibody against axonal neurofilament proteins and several antibodies against glycosaminoglycans including HS, dermatan sulfate, keratan sulfate, and HS‐proteoglycan. Ultrastructural localization of the immunoreactivity was also studied by using pre‐embedding immunoperoxidase and immunogold methods. On the basis of the findings of this study, only HepSS‐1‐recognized HS among several glycosaminoglycans is clearly detected in the spinal spheroids in MND, and it exists in close association with accumulated neurofilaments. Because of its capacity to bind various proteins, HS may act as a trigger or an accelerator for the accumulation of neurofilaments and may play important roles in the formation of spheroids in MND.