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An immunohistochemical study of Alzheimer neurofibrillary tangles using confocal laser scan microscopy
Author(s) -
Ohwada Jiro,
Nishiyama Etsuko,
Iwamoto Norihiko,
Arai Heii
Publication year - 1995
Publication title -
neuropathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 61
eISSN - 1440-1789
pISSN - 0919-6544
DOI - 10.1111/j.1440-1789.1995.tb00278.x
Subject(s) - neurofilament , confocal , pathology , confocal microscopy , immunohistochemistry , amyloid (mycology) , alzheimer's disease , neurofibrillary tangle , tau protein , chemistry , senile plaques , biology , medicine , microbiology and biotechnology , physics , optics , disease
Double immunofluorescent stainings of neurofibrillary tangles (NFT) were performed using anti‐tau, ubiquitin, amyloid P component and neurofilament antibodies. A confocal laser scan microscope was used to investigate the distribution and the relationship of these components. Tau‐ and ubiquitin‐immunoreactivities (IR) were observed in the same NFT. Tau‐ and neurofilament‐IR were observed in the same neuron. Tau‐ and amyloid P com‐ponent‐IR were not colocalized in the same NFT. These findings may suggest a process of NFT‐formation which will help in the understanding of pathophysiology of Alzheimer's disease.