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Urinary 17α‐hydroxyprogesterone in management of 21 ‐hydroxylase deficiency
Author(s) -
LIM Y. J.,
YONG A. B. W.,
WARNE G. L.,
MONTALTO J.
Publication year - 1995
Publication title -
journal of paediatrics and child health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.631
H-Index - 76
eISSN - 1440-1754
pISSN - 1034-4810
DOI - 10.1111/j.1440-1754.1995.tb02913.x
Subject(s) - medicine , urinary system , urine , congenital adrenal hyperplasia , morning , radioimmunoassay , endocrinology , immunoassay , chromatography , hydroxyprogesterone , column chromatography , immunology , chemistry , hormone , steroid , antibody
Objectives: The study was designed to assess the reliability of measurement of 24‐hour urinary 17α‐hydroxyprogesterone (17‐OHP) by radio‐immunoassay (RIA) as an alternative biochemical assessment for monitoring the treatment of congenital adrenal hyperplasia (CAH) due to 21 ‐hydroxylase deficiency (21 ‐OHD) and to assess the need for sample purification by column chromatography to improve assay specificity. Methodology: Morning serum 17‐OHP was measured using RIA and 24‐hour urinary pregnanetriol using gas chromatography. Twenty‐four‐hour urinary 17‐OHP was measured in samples from 17 prepubertal patients with CAH due to 21 ‐OHD, and 20 normal prepubertal children as controls. In 24 urine samples, RIA of 17‐OHP was performed with and without column chromatography. Results: There was a good correlation between 24‐hour urinary 17‐OHP and 24‐hour urinary pregnanetriol (r = 0.962, P <0.01) and between 24‐hour urinary 17‐OHP and morning serum 17‐OHP ( r = 0.955, P <0.01). There was no significant difference in the RIA of the urine samples with and without purification by column chromatography. Conclusions: The measurement of 24‐hour urinary 17‐OHP is a reliable alternative for the biochemical monitoring of 21‐OHD, and RIA specificity is unaffected by omission of column chromatography.