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Improved specificity of ELISA for rotavirus
Author(s) -
HOGG R. J.,
DAVIDSON G. P.
Publication year - 1982
Publication title -
journal of paediatrics and child health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.631
H-Index - 76
eISSN - 1440-1754
pISSN - 1034-4810
DOI - 10.1111/j.1440-1754.1982.tb02023.x
Subject(s) - medicine , rotavirus , complement fixation test , virology , false positive paradox , antibody , antigen , immunology , virus , serology , machine learning , computer science
. We have modified the ELISA assay for human rotavirus antigen detection so as to eliminate false positive reactions which occur due to extraneous antibodies. This has been accomplished by the incorporation of a control serum which contains no rotavirus specific antibodies, detectable by complement fixation, into the ELISA assay. Using this method, 9.7% of specimens giving positive results by the standard ELISA method as described by other workers, can be recognized as false positives and discarded.