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Enzyme linked immunosorbent assay for detecting specific IgM antibody in congenital cytomegalovirus infection
Author(s) -
ROBERTSON PETER W.,
KERTESZ VERA E.
Publication year - 1982
Publication title -
journal of paediatrics and child health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.631
H-Index - 76
eISSN - 1440-1754
pISSN - 1034-4810
DOI - 10.1111/j.1440-1754.1982.tb02022.x
Subject(s) - medicine , antibody , cytomegalovirus , virology , enzyme , immunoglobulin m , immunology , immunoglobulin g , herpesviridae , viral disease , virus , biochemistry , chemistry
. An enzyme linked immunosorbent assay (ELISA) was used to determine whether cytomegalovirus (CMV) specific IgM antibody was present in the serum of 135 neonates suspected of having congenital CMV infection. The assay was compared with the fluorescent antibody (FA) technique for cytomegalovirus specific IgM using the IgM fraction separated from serum by chromatography, a technique which has been shown to be a sensitive and specific method of detecting CMV specific IgM antibody. Elevated optical density reactions with the ELISA technique were only obtained with the seven sera that were shown to contain CMV specific IgM antibody by the fluorescent antibody technique. Sera from 128 neonates that were negative by the CMV‐IgM‐FA technique gave low optical density ELISA readings. The ELISA appeared at least as sensitive and specific as the fluorescent antibody test for detecting cytomegalovirus specific IgM antibody in neonates.