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Non‐invasive estimation of liver fibrosis in non‐alcoholic fatty liver disease using the 13 C‐caffeine breath test
Author(s) -
Park Gordon JH,
Wiseman Elke,
George Jacob,
Katelaris Peter H,
Seow Francis,
Fung Caroline,
Ngu Meng C
Publication year - 2011
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2011.06760.x
Subject(s) - medicine , gastroenterology , steatosis , steatohepatitis , fatty liver , cirrhosis , odds ratio , fibrosis , liver function , confidence interval , liver function tests , breath test , disease , helicobacter pylori
Background and Aim:  Fibrotic progression in non‐alcoholic fatty liver disease (NAFLD) is associated with impaired hepatic function. The 13 C‐caffeine breath test (CBT) is a non‐invasive, quantitative test of liver function. We sought to determine the utility of the CBT in detecting hepatic fibrosis in NAFLD. Methods:  The CBT was applied to 48 patients with NAFLD. CBT results were compared to clinical, biochemical and histological data. Twenty‐four healthy subjects served as controls. Results:  Patients with simple steatosis had similar CBT values (2.28 ± 0.71 Δ‰ per 100 mg caffeine) to controls (2.31 ± 0.85, P  = 1.0). However, CBT was significantly reduced in patients with non‐alcoholic steatohepatitis (1.59 ± 0.65, P  = 0.005) and cirrhosis (1.00 ± 0.73, P  < 0.001). CBT significantly correlated with Brunt's fibrosis score ( r  = −0.49, P  < 0.001) but not with steatosis ( P  = 0.23) or inflammation ( P  = 0.08). CBT also correlated with international normalized ratio ( r  = −0.61, P  < 0.001), albumin ( r  = 0.37, P  = 0.009), aspartate aminotransferase/alanine aminotransferase ( r  = −0.34, P  = 0.018) and platelets ( r  = 0.31, P  = 0.03). On multivariate analysis, age (odds ratio 1.12, 95% confidence interval 1.042–1.203, P  = 0.002) and CBT (OR 0.264, 95% CI 0.084–0.822, P  = 0.02) were independent predictors of significant fibrosis ( F  ≥ 2). CBT yielded an area under the receiver operating characteristic curve of 0.86 for the diagnosis of cirrhosis. Conclusions:  The CBT reflects the extent of hepatic fibrosis in NAFLD and represents a non‐invasive predictor of fibrosis severity in this condition.

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