Premium
LMP2 / LMP7 gene variant: A risk factor for intestinal Mycobacterium tuberculosis infection in the Chinese population
Author(s) -
Lv Yuan,
Yan Bo,
Yang Hongli,
Liu Jian,
Zhong Wei,
Li Kun,
Chen Ziping,
Xu Changqing
Publication year - 2011
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2011.06693.x
Subject(s) - tuberculosis , mycobacterium tuberculosis , haplotype , genotype , immunology , medicine , single nucleotide polymorphism , odds ratio , virology , gene , biology , genetics , pathology
Background and Aims: Low molecular mass protein‐2 ( LMP2 ) and low molecular mass protein‐7 ( LMP7 ) genes play a critical role in foreign antigen processing on the major histocompatibility complex‐I CD8 + cytotoxic T‐lymphocyte pathway. This study was designed to investigate whether the sequence variants in the LMP2 / LMP7 coding region were associated with intestinal Mycobacterium tuberculosis ( M. tuberculosis ) infection or with the co‐infection of pulmonary tuberculosis. Methods: A total of 168 patients with intestinal tuberculosis and 235 normal controls were recruited for this study. Two polymorphisms of LMP2 (Arg60–His) and LMP7 (Gln145–Lys) were identified by polymerase chain reaction–restriction fragment length polymorphism method. The associations of the LMP2 / LMP7 genotype and haplotype with intestinal M. tuberculosis infection were assessed by using logistic regression analysis. Results: The results revealed that LMP7 position codon 145 Lys/Lys and Gln/Lys alleles in the coding region were associated with the infection of intestinal M. tuberculosis ( P = 0.003, odds ratio [OR] = 3.86 and P < 0.001, OR = 2.28, respectively). Meanwhile, the Arg–Lys and Cys–Lys haplotypes exhibited significant relation to the intestinal M. tuberculosis infection ( P = 0.006, OR = 1.87; P = 0.021, OR = 1.83, respectively). No significant associations were observed for any of the single‐nucleotide polymorphism genotypes or haplotypes with the co‐infection of pulmonary tuberculosis ( P > 0.05). Conclusions: The results indicated that the genetic variant within the LMP2 / LMP7 gene would increase the risk of intestinal M. tuberculosis infection.