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Association of cytotoxic T lymphocyte associated antigen‐4 gene (rs60872763) polymorphism with Crohn's disease and high levels of serum sCTLA‐4 in Crohn's disease
Author(s) -
Chen Zhitao,
Zhou Feng,
Huang Sha,
Jiang Ting,
Chen Liping,
Ge Liuqing,
Xia Bing
Publication year - 2011
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2011.06662.x
Subject(s) - medicine , immunology , crohn's disease , odds ratio , allele , cytotoxic t cell , ctla 4 , antigen , gastroenterology , inflammatory bowel disease , disease , t cell , gene , immune system , biology , genetics , in vitro
Background and Aim: Our aim was to evaluate cytotoxic T lymphocyte associated antigen‐4 (CTLA‐4) gene polymorphisms in Crohn's disease (CD) and explore soluble CTLA‐4 (sCTLA‐4) levels in serum of CD patients in central China. Methods: A total of 126 Chinese CD patients and 300 healthy controls were enrolled in this study. CTLA‐4 (AT)n repeat polymorphism was genotyped by a semiautomatic fluorescently labeled polymerase chain reaction (PCR) method, and CTLA‐4 ‐1661A/G and ‐1722T/C polymorphisms were genotyped by DNA sequencing. Serum sCTLA‐4 and C‐reactive protein (CRP) levels were determined by enzyme linked immunosorbent assay (ELISA) and immunonephelometry, respectively. Results: The frequency of 84 bp allele of CTLA‐4 (AT)n repeats was lower in CD patients than in the healthy controls (22.2% vs 33.2%, P = 0.001, odds ratio = 0.58, 95% confidence interval: 0.41–0.81). The 84 bp allele carriers of (AT)n repeats were associated with non‐stricturing and non‐penetrating disease behavior in CD patients ( P = 0.007). Serum sCTLA‐4 levels were more elevated in CD patients than in the healthy controls ( P < 0.001). Among CD patients, serum sCTLA‐4 levels were increased in active disease compared with inactive disease ( P = 0.015), and were correlated with CRP levels ( r = 0.524, P < 0.001). Serum sCTLA‐4 levels were higher in CD patients with stricturing disease behavior than in patients with other disease behaviors ( P = 0.009). Conclusions: 84 bp allele of CTLA‐4 (AT)n repeat polymorphism was associated with CD in central China. sCTLA‐4 levels were highly expressed in CD, especially in active disease, and were correlated with CRP levels and disease behavior in CD patients.